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多克隆抗体

GFAP Antibody

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产品详情相关产品

GFAP Antibody

货号：

HYK-200116
规格：
图片：
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate
All lanes: GFAP antibody at 3.9µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 50, 51 kDa
Observed band size: 50 kDa

IHC image of CSB-PA009369LA01HU diluted at 1:200 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA009369LA01HU diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of A549 cells with CSB-PA009369LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

产品详情

产品名称：

Rabbit anti-Homo sapiens (Human) GFAP Polyclonal antibody
Uniprot No.：

P14136
基因名：

GFAP
别名：

GFAP antibody; GFAP Epsilon antibody; GFAP_HUMAN antibody; GFAPdelta antibody; GFAPepsilon antibody; Glial fibrillary acidic protein antibody; Intermediate filament protein antibody
宿主：

Rabbit
反应种属：

Human
免疫原：

Recombinant Human Glial fibrillary acidic protein (292-432AA)
免疫原种属：

Homo sapiens (Human)
标记方式：

Non-conjugated
克隆类型：

Polyclonal
抗体亚型：

IgG
纯化方式：

>95%, Protein G purified
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
产品给予形式：

Liquid
应用范围：

ELISA, WB, IHC, IF

推荐稀释比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.