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语言：Eng
重组抗体
- Acetyl-Histone H3.1(K56)Antibody
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凯发K8旗舰生物抗体平台现已拥有4万多种抗体(含单抗和多抗)，包括人类常用抗体，模式生物类相关抗体、小分子抗体、二抗、标签抗体及内参抗体等。并以每年4000种的速度在持续增长。
凯发K8旗舰生物拥有专业的质控团队，配备先进实验仪器，现已成功地建立了ChIP平台，因此我们的抗体不仅能给予Biotin、FITC、HRP等标记，大部分抗体都能进行ELISA、WB、IHC、IF、IP、ChIP等应用验证。
我们致力于为所有用户给予高亲和力、高特异性的优质抗体，欢迎来电咨询。
Acetyl-Histone H3.1(K56)Antibody
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货号：HYK-200281
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规格：
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图片：
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Western Blot
Positive WB detected in:Hela whole cell lysate treated by 15mM sodium butyrate for 30min
All lanes:Acetyl-Histone H3.1(K56)antibody at 0.7μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 15 KDa
Observed band size: 15 KDa
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Immunocytochemistry analysis of CSB-RA010418A56acHU diluted at 1:100 and staining in HepG2 cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with CSB-RA010418A56acHU at 1:43,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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产品详情
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Uniprot No.：P68431
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基因名：HIST1H3A
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别名：H3 histone family member E pseudogene antibody; H3 histone family; member A antibody; H3/A antibody; H31_HUMAN antibody; H3F3 antibody; H3FA antibody; Hist1h3a antibody; HIST1H3B antibody; HIST1H3C antibody; HIST1H3D antibody; HIST1H3E antibody; HIST1H3F antibody; HIST1H3G antibody; HIST1H3H antibody; HIST1H3I antibody; HIST1H3J antibody; HIST3H3 antibody; histone 1; H3a antibody; Histone cluster 1; H3a antibody; Histone H3 3 pseudogene antibody; Histone H3.1 antibody; Histone H3/a antibody; Histone H3/b antibody; Histone H3/c antibody; Histone H3/d antibody; Histone H3/f antibody; Histone H3/h antibody; Histone H3/i antibody; Histone H3/j antibody; Histone H3/k antibody; Histone H3/l antibody
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反应种属：Human
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免疫原：A synthesized peptide
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免疫原种属：Homo sapiens (Human)
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标记方式：Non-conjugated
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克隆类型：Monoclonal
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抗体亚型：Rabbit IgG
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纯化方式：Affinity-chromatography
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浓度：It differs from different batches. Please contact us to confirm it.
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保存缓冲液：Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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产品给予形式：Liquid
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应用范围：ELISA, WB, ICC, IF
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推荐稀释比：
Application Recommended Dilution WB 1:500-1:2000 ICC 1:50-1:500 IF 1:30-1:200 -
Protocols：ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
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储存条件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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货期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
CD86 Antibody
CD21 Antibody
Acetyl-Histone H2B type 1-B(K20)Antibody
Di-methyl-Histone H3.1(K9)Antibody
Histone H2B type 1-K Antibody
Histone H4 Antibody
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