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语言：Eng
重组抗体
- CD146 Antibody
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凯发K8旗舰生物抗体平台现已拥有4万多种抗体(含单抗和多抗)，包括人类常用抗体，模式生物类相关抗体、小分子抗体、二抗、标签抗体及内参抗体等。并以每年4000种的速度在持续增长。
凯发K8旗舰生物拥有专业的质控团队，配备先进实验仪器，现已成功地建立了ChIP平台，因此我们的抗体不仅能给予Biotin、FITC、HRP等标记，大部分抗体都能进行ELISA、WB、IHC、IF、IP、ChIP等应用验证。
我们致力于为所有用户给予高亲和力、高特异性的优质抗体，欢迎来电咨询。
CD146 Antibody
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货号：HYK-200100
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规格：
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图片：
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Western Blot
Positive WB detected in:A375 whole cell lysate
All lanes:CD146 antibody at 0.6μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 72 KDa
Observed band size: 120 KDa
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IHC image of CSB-RA013563A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
Immunofluorescence staining of U251 cells with CSB-RA013563A0HU at 1:37,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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产品详情
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Uniprot No.：P43121
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基因名：MCAM
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别名：A32 antigen antibody; CD 146 antibody; CD146 antibody; CD146 antigen antibody; Cell surface glycoprotein MUC18 antibody; Cell surface glycoprotein P1H12 antibody; Gicerin antibody; Mcam antibody; Melanoma adhesion molecule antibody; Melanoma associated antigen A32 antibody; Melanoma associated antigen MUC18 antibody; Melanoma associated glycoprotein MUC18 antibody; Melanoma cell adhesion molecule antibody; Melanoma-associated antigen A32 antibody; Melanoma-associated antigen MUC18 antibody; MelCAM antibody; MUC 18 antibody; MUC18 antibody; MUC18_HUMAN antibody; S endo 1 antibody; S endo 1 endothelial associated antigen antibody; S-endo 1 endothelial-associated antigen antibody
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反应种属：Human
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免疫原：A synthesized peptide
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免疫原种属：Homo sapiens (Human)
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标记方式：Non-conjugated
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克隆类型：Monoclonal
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抗体亚型：Rabbit IgG
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纯化方式：Affinity-chromatography
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浓度：It differs from different batches. Please contact us to confirm it.
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保存缓冲液：Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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产品给予形式：Liquid
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应用范围：ELISA, WB, IHC, IF, FC
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推荐稀释比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:50-1:500 IF 1:30-1:200 -
Protocols：ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
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储存条件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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货期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or , please kindly consult your local distributors for specific delivery time.
N Antibody
SARS-CoV-2 Spike RBD Nanobody, HRP conjugated
SARS-CoV-2 Spike RBD Nanobody, FITC conjugated
SARS-CoV-2 Spike RBD Nanobody, Biotin conjugated
Phospho-Histone H3.3 (T3) Antibody
Di-methyl-Histone H3.1(K4)Antibody
在线询价
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